This positive control is a validated gRNA sequence targeting RELA with editing efficiency of 80% - 90% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency with SpCas9.
Includes 1 nmol of modified sgRNA.
This positive control is a validated gRNA sequence targeting CDC42BPB with editing efficiency of 80% - 95% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency with SpCas9.
Includes 1 nmol of modified sgRNA.
Negative control gRNAs are designed to not target any genomic location in the human & mouse genome.
This gRNA generates a non-edited control for analyzing SpCas9-edited cells.
Includes 1 nmol of modified sgRNA.
Negative control gRNAs are designed to not target any genomic location in the human & mouse genome.
This gRNA generates a non-edited control for analyzing SpCas9-edited cells.
Includes 1 nmol of modified sgRNA.
This positive control is a validated gRNA sequence targeting Rosa26 in mouse cell lines. Use this control to optimize transfection conditions for maximum editing efficiency with SpCas9.
Includes 1 nmol of modified sgRNA.
This multi-guide positive control is a validated trio of gRNAs targeting TRAC in human cell types.
For the full kit of the positive control with the addition of sequencing and PCR primers as well as Cas9 nuclease, purchase the Transfection Optimization Kit.
Includes 1.5 nmol of modified multi-guide sgRNA.
This validation-ready controls kit is engineered specifically for the high-fidelity eSpOT-ON nuclease system. It facilitates precise calibration of gene editing workflows by providing optimized positive and negative gRNA benchmarks. These controls are critical for validating delivery protocols, assessing cleavage efficiency, and ensuring accurate normalization of on-target indel formation data.
This validated controls kit is engineered for SpCas9 gene editing in human cells. It enables the precise assessment of cleavage efficiency and specificity by providing benchmark positive and negative gRNAs targeting a known locus. These controls are essential for optimizing transfection or electroporation protocols, validating sgRNA delivery, and quantifying on-target indel formation for reliable data normalization.
Formulated with 10 mM Tris-HCl and 1 mM EDTA at pH 8.0, this TE Buffer protects nucleic acids from degradation during long-term storage. Ideal for solubilizing nucleic acids, diluting samples, and washing precipitates in molecular biology protocols.
Prepared for critical molecular biology workflows, this nuclease-free water ensures RNA stability. Validated for use in CRISPR gRNA preparation, RNA extraction, cDNA synthesis, and ligation.
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